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. 4 strongly supported the conclusion that pretransitional conformation changes occurred at temperatures where the enzyme retained high activity (see Fig
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. In the whole pH range explored, the absolute values of the [Θ] at 222 nm showed a bell-shaped tendency (Fig
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. Furthermore, in some cases the differences in intensity signals from folded and unfolded states are very small, which makes precise measurements difficult (12)
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. X-ray crystal structure of the two site-specific mutants Ile7Ser and Phe110Ser of Azurin from Pseudomonas aeruginosa
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. TheoryThe equations for the relaxation of a backbone amide ^sup 15^N spin in a protein as well as their interpretation in terms of the model-free approach of Lipari and Szabo (25) are well described in the literature (see, e
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. This indicates that the Bis-ANS molecule is too big to completely fit in the thyroxine binding cavity and likely there is only room for one-half of the symmetric molecule
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. Further work seems here necessary, and fluorescence probes exhibiting longer decay time is highly desirable for direct determination of the size distribution of the oligomer assemblies and protofibrils
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. 4, we show that Gα^sub s^ is destabilized under pressure even at relatively high lipid concentrations, although we note that membrane binding of Gα^sub s^ is weaker than Gβγ (i
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. This precisely exemplifies the general mechanism we have suggested, with the additional feature that one of the hypergutters has become so stabilized (and therefore so populated) that it attracts the label of "intermediate state"
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. Isotactic polypropylene (IPP) and poly(ethylene-co-vinyl acetate) (EVA) are converted into toners by means of TIPS
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. This indicated that most of the Trp residues in ACYI were buried in the interior (Class I) or exposed to bonded water (Class II), and no Trp residues were in a highly hydrophobic (Class A and S) or fully solvent-exposed (Class III) microenvironment
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.Attributes of Glycosylation in the Establishment of the Unfolding Pathway of Soybean AgglutininSinha, SharmisthaABSTRACT Soybean agglutinin (gSBA) is a tetrameric legume lectin, each of whose subunits are glycosylated
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. The conformations, which are drawn at random from the ensembles, are oriented in the principle axis frames to illustrate the average prolate ellipsoidal shape as well as the large fluctuations that characterize the conformational distributions
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. Gradient, high-resolution, magic angle spinning ^sup 1^H nuclear magnetic resonance spectroscopy of intact cells
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. Secreted DmAChE was purified to homogeneity using the following steps: 70% ammonium sulfate precipitation, dialysis, affinity chromatographies on a procainamide-Sepharose column and on nitrilotriacetic acid-nickel agarose (39)
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. Reference spectra of conformational limits
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. Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Forster distance dependence and subpopulations
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." Annals of Pharmacotherapy 36: 1686-91
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