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. The use of KI yielded similar results at both excitation wavelengths (Table 1): the values of K^sub sv^ were larger at acidic pHs and in GdmHCl than at neutral pH
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. Although for BSA a high binding capacity may be expected from its intrinsic tendency to bind nonpolar and aromatic moieties also in the liquid state, the above ANS binding data do suggest that beyond specific trends of individual polypeptides, multi-subunit proteins are more susceptible than monomers to destabilization and partial unfolding in the frozen state
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. Quantitative estimation of protein binding site polarity
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. This property is only related to the chemical nature of the flux and additives and is independent of the solid contents of the flux
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. Fluorescence spectroscopy provides a very attractive alternative for such studies, due to sensitivity, ease of use, and flexibility
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. This produces unfolded monomeric TTR
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. It is notable that the saturated palmitoyl chains would be expected to become more stabilized as the membranes enter the gel phase, but the opposite is observed
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. Instead they have served just to keep the diffusive search within the smaller (d - 1) or (d - 2) dimensional space, once the first subdimensional search is over, then within a (d - 3) or (d - 4) dimensional space after the subsequent successful "adsorption" into the still-smaller subspace, and so on
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. However, ages have been validated for yellowtail rockfish (Sebastes flavidus), a closely related species (Leaman and Nagtegaal, 1987), by using otoliths; moreover, the break-and-burn aging method is widely accepted as valid for aging rockfish (MacLellan, 1997), and ages thus derived are routinely used in rockfish stock assessments
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. Seasonal histological changes in the gonads of Sebastodes paucispinis (Ayres), and ovoviviparous teleost (family Scorpaenidae)
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. In An atlas of the early stage fishes in Japan (M
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. For histogram simulations of the SL 4WJ at 10 mM Mg^sup 2+^, the state of the ribozyme system was allowed to stochastically switch between the extended undocked and quasi-docked states according to the assumed forward and reverse rate constants (see Fig
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. Hence, the 4WJ ribozyme is unique in its ability to fold into a structure with arms A and B in close proximity, similar to the native ribozyme, even in the absence of specific loop-loop docking interactions
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. Buckwalter JA, Mankin HJ
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. The concentration of ANS, as determined by absorbance at 370 nm, using ε^sub 370^ = 6800 M^sup -1^Cm^sup -1^ (in methanol), ranged between 10 and 450 µM although in most experiments the upper limit was 320 µM
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