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Thermal unfolding of ribonuclease A in phosphate at neutral pH: deviations from two-state model
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Asymmetry in the shapes of folded and denatured states of proteins
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This shoulder seems to represent a subpopulation of thermal unfolded states which exhibit less buried tryptophan residues, possibly related to unfolded proteins which are not aggregated
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As for azurin (12), lowering the freezing temperature from -5°C to -13°C increases the number of binding sites but the average affinity is lowered
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Sample preparation for neutron scatteringA batch of ~250 mg of HuBChE was dialyzed against 25 mM ammonium acetate
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For AHS both approximate analytic (71) and recent machine calculations (20) indicate that to a very large extent the metastable liquid-liquid coexistence curve lies below the percolation line, and so particle gel formation rather than liquid-liquid separation is predicted
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The remaining charge of DNA is neutralized by other positively charged chemicals in cell fluid, where mono- and divalent cations comprise up to 1% of the cell weight
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Eocating monovalent cations in the grooves of B-DNA
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The evident binding of these four different dyes to misfolded conformations of TTR indicates their potential use as tools for following the misfolding and aggregation process
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We compared the properties of ThT with that of DCVJ and found a very similar dependence on viscosity after the increasing relative concentration of glycerol (Fig
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Studies using mutants are underway to better identify the residues responsible for these interaction volumes
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02]);* The body was rather elongate instead of moderate (BD ranged from 0
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By combining the trajectories of the free and complex forms, further insight into the conformational sampling was extracted
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Appending the complex trajectory of gp120 to the free one gives rise to a small increase (ΔS^sup 2nd,f+c^^sub CD4,F43^ = 0
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"Scar treatment demands time and consistency
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For Professor Croce, these alternatives are the truly scientific methods of biomedical research as opposed to the pseudo-science of vivisection
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, over age 21 years) who are typically reported to meet the criteria (APA, 1994)
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Journal of Consulting and Clinical Psychology, 66, 474-483
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