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. For instance, we have shown that the thennodynamic folding parameters (ΔC^sub p^ and m-values) of the HPr of S
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. Am J Respir Cell Mol Biol 2006; 34:274-285(44) Botto M, Dell'Agnola C, Bygrave AE, et al
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.193 K/ min (from the apparent T^sub m^ = 84°C to 75°C)
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. Apart from the faster kinetics, the increased temperature leads to a more complex morphological variability: long straight rods, twisted ribbonlike structures, rod bundles, and ropelike structures become prominent in the corresponding AFM data
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. However, a quantitative relation between the topomer search space and contact order is still unclear, since no native contacts are actually required to form at a purely topologically-defined transition state at all (although many are to be expected from the partial ordering at the secondary level at least)
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.Features of the time-dependent folding curves as functions of temperature or denaturant also follow from the model, including the possibility of an apparent delay before single exponential kinetics set in
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