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. Most of these difficulties are not present if λ^sub max^-signals are employed to characterize unfolding transitions, although these signals can give a skewed assignment of the population of microstates, because a priori the λ^sub max^-signal is not proportional to the population of the states
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. In both cases, the rate of increase of the MSDs decreases drastically at the highest measured temperatures (T > 270 K), as observed previously (44)
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- " As this article goes to press, more protest resignations, this time from the Carter Center's board of councilors, appear to be in the works
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.To characterize in more detail the structural changes induced by GdnHCl, we have investigated the accessibility of thiol groups of GR (which contains six Cys residues per subunit) to modification with DTNB (Fig
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. The amide I' region of native ACYI was dominated by bands from the α-helix (1651 cm^sup -1^), α-helix and/or 3 m helix (1660 cm^sup -1^), β-sheet (1629, 1637, and 1682 cm^sup -1^), β-turn (1668 and 1674 cm^sup -1^), and random coil (1644 cm^sup -1^)
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. A novel subset of amidohydrolases and insights into the enzyme mechanism
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. Thermal melts done with both rSBA and gSBA were found to he irreversible in nature, and visible aggregates were noticed at the end of the scans in either case
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.Biophysical Characterization of the Enzyme I of the Streptomyces coelicolor Phosphoenolpyruvate:Sugar Phosphotransferase SystemHurtado-Gómez, EstefaníaABSTRACT The first protein in the bacterial phosphoenolpyruvate (PEP):sugar phosphotransferase system is the homodimeric 60-kDa enzyme I (EI), which autophosphorylates in the presence of PEP and Mg^sup 2+^
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. In vitro mutagenesis and expression in Xenopus oocytes
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.DISCUSSIONThe influence of the chemical nature of a monocation on its DNA compaction ability is clearly demonstrated by the observation of the collapse of single DNA molecules by fluorescent microscopy
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. 3; for details see Hsu and Bonvin, 2004), only the backbone configurational entropy starts to level off after ~4 ns (Fig
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.A Curious Pellet From a Great Horned Owl (Bubo Virginianus)Woodman, NealAbstract -One of the traditional methods of determining the dietary preferences of owls relies upon the identification of bony remains of prey contained in regurgitated pellets
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