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. Spectra were averaged over three scans for each sample containing 3 µM GR in 30 mM HEPES, at pH 7
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. 4, s^sub i^ = (r^sub i^ - r^sub CM^), where r^sub CM^ is the position vector of the center of mass and r^sub i^ denotes the position vector of the α-carbon for residue i
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. J Immunol 2006; (in press)(57) Lee JH, Lee DS, Kim EK, et al
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. Spatial localization in NMR spectroscopy in vivo
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. Approximately half of the HuBChE was inhibited with soman, as described by Masson and Goasdoue (35), before dialysis and lyophilization
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. Bis-ANS was omitted from this binding study due to the promiscuous behavior in terms of fluorescence response; it essentially binds to any conformational state of TTR
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. , before the onset of the second calorimetric transition, is fully reversible and leads to no detectable changes in the subsequent DSC scans (data not shown)
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. The T-jump data to 95°C are well approximated by a single exponential, yet two exponents are required to fit the data at 75-90°C, suggesting two kinetic phases
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. After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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. Apolipoprotein specificity for lipid efflux by the human ABCAI transporter
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. Denaturant-induced movement of the transition state of protein folding revealed by high-pressure stopped-fiow measurements
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