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. Evidence for the existence of an unfolding intermediate state for aminoacylase during denaturation in guanidine solutions
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. 7, d-j, gives a pictorial view of the glycanprotein interactions
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. Of particular interest is the question of how preferential interactions in 8 M urea or 6 M GdnCl conspire to make these conditions mimic perfect solvents for proteins
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. The final concentrations were 5 mM MgCl^sub 2^ and 2
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. Fluorescent labeling and measurementsProteins were labeled with 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM)
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. Similar unfolding below 25°C was detected in the earlier studies of human lipid-free apoA-2 at much higher protein concentration (>0
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. Mechanism of dissociation of human apolipoprotein A-1 from complexes with DMPC as studied by guanidine hydrochloride denaturation
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. Docking of the two loops is also observed in simpler ribozyme constructs comprised of two- or three-way helical junctions (2WJ and 3WJ, respectively), although much higher Mg^sup 2+^ concentrations are required (N
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. For comparison, we also overlay histograms for twofold slower and faster rate constants (dashed lines), with the equilibrium constant maintained at 3
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. Among them, only 11 eggs were identified as Callionymoidei spp
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., at the lowest Mg^sup 2+^ concentration
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. Effect of transforming growth factor-[beta] on proteoglycan synthesis by chondrocytes in relation to differentiation stage and the presence of pericellular matrix
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