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Protein concentration used for isothermal melts in lhe fluorimeler was 0
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An example of this comparison is shown in Fig
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13 shows a comparison of P(x) predicted by theory to distributions computed for four different proteins in the EV limit
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In less-polar environments of the tryptophan chromophore the stokes shift will be less pronounced as compared to more-polar surroundings
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Their main utility is to compare differences in the ANS binding propensity among proteins or brought about by changing experimental conditions such as ice temperature, freezing protocols, or the addition of solutes
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The static magnetic field dependence of chemical exchange linebroadening defines the NMR chemical shift time scale
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The inverse relationship between compaction and binding activities can be explained as follows
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The insulin aggregation is promoted under conditions favoring partly destabilized monomers and dimers, such as low pH, high temperature, and contact with hydrophobic media (e
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Particle agglomerates were broken up using first a blender (Osterizer 10-speed blender), and then a jet mill (Eriez, model HI-VI, Erie, PA) operated at room temperature after sieving through a 100 µm metal sieve
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Additional funding was provided through Hatfield Marine Science Center scholarships: the 1996 Barbara Schwantes Memorial Fellowship; the 1997 Mamie L
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The 2 × 2 [chi]^sup 2^ method (a bivariate type of analysis with 1 degree of freedom) was used to determine significance
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16 Re-expression of a differentiated phenotype is enhanced by the presence of defined growth factors, such as TGF-[beta], insulin or IGF-I
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When the player lacked control of the dealer, "Dealer is Andrew" was displayed against a green background at the center of the screen
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No other effects were significant
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. Even today, the question has no definitive answer--though several fascinating lines of research suggest a genuinely Darwinian resolution is within sight
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. Although the thermal unfolding of many proteins can successfully be illustrated by a two-state model, step-wise transitions have been observed in several proteins regardless of whether the process was reversible or irreversible (30,38, 39,49-54)
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. pH-induced structural changes Steady-state intrinsic fluorescence measurements
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. Lipoxin enhances efferocytosis in vitro (54) and suppresses the inflammatory response, and its levels are low in CF patients
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. For this purpose we analyzed the unfolding transitions of the medium-sized multitryptophan protein α-amylase mainly by the use of λ^sub max^-signals
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. Copper-free azurins (apoazurins) were prepared from holo-azurins by adding potassium cyanide and EDTA to final concentrations of 0
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. DCVJ was excited at 470 nm and ThT at 440 nm
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. were recorded at 222 nm during sample heating and cooling from 5-98°C at various scan rates from 1
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. Myomere and fin-ray counts and morphometric measurements were made on the left side of the body
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.23 kJ K^sup -1^ mol^sup -1^
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. This suggests that, for these sets, sufficient sampling of conformational space has been achieved and that relatively precise conformational entropy differences between the two states can be obtained, although the values for the V3 loop should be taken with caution
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.The peak position for the SL 3WJ remains in the low-FRET region of the histograms, independent of the Mg^sup 2+^ concentration, indicating that this ribozyme construct is incapable of folding to a compact structure (Fig
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