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. The inclusion bodies thus obtained were precipitated using 65% ice-cold acetone
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. INTRODUCTION The bacterial phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) is a multiprotein complex system that phosphorylates and, concomitantly, transports carbohydrates across the membrane into the cell (1)
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. 002 µg/µl for trypsin, that is, the final concentration rate between both proteins was 500:1) in a total volume of 17 µl in 100 mM of Tris buffer at pH 7 (27)
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. Molten globule and protein folding
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. A schematic representation of the TTR misfolding reaction from the unfolded state through the A-state is presented in Fig
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. This is both natural and necessary, since data from kinetic experiments do just the same, but there is a danger in overlooking aspects of folding that rely essentially on the presence of many degrees of freedom
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. Research elsewhere [1, 10, 12, 13] has shown that such gels form with a thermal phase inversion of polypropylene solutions in a hydrocarbon solvent when entering the metastable region of the phase diagram upon cooling
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