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. The amide I' region of native ACYI was dominated by bands from the α-helix (1651 cm^sup -1^), α-helix and/or 3 m helix (1660 cm^sup -1^), β-sheet (1629, 1637, and 1682 cm^sup -1^), β-turn (1668 and 1674 cm^sup -1^), and random coil (1644 cm^sup -1^)
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. Manuscript received February 14, 2006; revision accepted March 24, 2006
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. Osmotic equilibria in solutions of serum albumin and sodium chloride
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. Assignment of the contribution of the tryptophan residues to the circular dichroism spectrum of human carbonic anhydrase II
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. Therefore, we characterized the membrane binding behavior of PLCβ and G-protein subunits before protein-protein association studies
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. We find that the K^sub d^ value for this interaction in solution is higher than expected based on a simple model where we calculate protein association on the reduced volume of the membrane
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. We note here that although we have interpreted peak shifts and broadening in terms of distance changes and ribozyme subpopulations, respectively, peak shifts could also potentially be caused by solvent effects on dye photophysics, and corresponding changes in the correction factor [gamma] (see Materials and Methods), as well as peak broadening by effects of [kappa]^sup 2^, the orientation factor (Deniz et al
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. A collapsed non-native RNA folding state
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- The money will fund a number of ministries, including an "adopted" public school in the South Bronx
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. Protein concentration was 4 µM
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. Approximately half of the HuBChE was inhibited with soman, as described by Masson and Goasdoue (35), before dialysis and lyophilization
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. Residues 14, 19, 51, 55, 76, 92, and 118 show a relatively strong decrease in exchange rate upon change in temperature (f = 0
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. This approach requires that the pH be raised to 7
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., 1999; Plaxco and Baker, 1998; Goldberg and Baldwin, 1995)
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. Folding dynamics of the B1domain of protein G explored by ultrarapid mixing
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