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Stronger DocumentationRecoveries from borrowers often are unavailable because borrowers may be victimized themselves
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For instance, we have shown that the thennodynamic folding parameters (ΔC^sub p^ and m-values) of the HPr of S
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Interdomain interaction and substrate coupling effects of dimerizalion and conformational stability of Enzyme I of the Esclierichia coli phosphoenolpyruvate:sugar phosphotransferase system
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Apoptotic cells are rarely seen in the nondiseased human lung, (3) suggesting either low rates of cell death or a rapid removal process
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When we examined BAL fluid from COPD patients, we found increased numbers of apoptotic cells and decreased numbers of apoptotic cell ingestions, which is consistent with an efferocytosis defect (W
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Nat Genet 1998; 19:56-59(45) Baumann I, Kolowos W, Voll RE, et al
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To look at the generality of the phenomenon, ANS binding experiments were carried out on seven new proteins, namely, monomers (bovine serum albumin (BSA), α-amylase, and β-galactosidase (β-gal)), a dimer (alcohol dehydrogenase from horse liver (LADH)), and tetramers (alcohol dehydrogenase from yeast (YADH), lactic dehydrogenase (LDH), and aldolase (ALD))
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Thus, the N-H^sup N^ bond vectors within an α-helix must have similar (τ^sup o^^sub m^)^sub app^, and the average (τ^sup o^^sub m^)^sub app^ for each helix can be taken to reduce the error
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Such prefibrillar oligomers could also serve as a common intermediate between the amyloidoses and other misfolding diseases, including prion diseases, Parkinson's disease, and amytrophic lateral sclerosis
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The entropie effect may result from the contribution of the cratic (mixing) entropy of protein dissolution to the entropy of disk denaturation
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5 a, height and in Fig
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The donor and acceptor signals were further filtered using an HQ585/60m band-pass filter (donor, Chroma Technology Corp
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. To avoid misleading results from the heavy overlapping of the original IR amide I' band, FSD data, which maintained the intensity of the original data, were used for the phase diagram analysis
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. A total of 27 hydrogen-bonding interactions and 53 hydrophobic interactions were found to stabilize the structure, which suggests that hydrophobic interactions between the protein and the glycans are responsible for the exceptionally high stability of the glycoprotein
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. This widelycited result provides the theoretical basis for the assertion that denatured proteins are Flory-like random coils (3,4,11,12,17,37,30)
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. Fifty years of solvent denaturation
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. At basic pHs, the K^sub sv^s were similar, within the error, to those observed at pH 7
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. Similarly, collectin family members, including mannose-binding lectin, surfactant protein (SP)-A, SP-D, and the collectin-like Clq, opsonize apoptotic cells with their globular heads, and then form a complex through their collagenous tails with phagocyte calreticulin and LRP
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. Although suitable data are not available to establish if there is a direct correlation between the above ANS binding capacity and the actual instability to freeze-thawing (depending on the protein, irreversible damage has been estimated by diverse criteria such as loss of enzymatic activity, degree of aggregation, and loss of secondary or tertiary structure and, further, the outcome is generally influenced by the rates of freezing and thawing), it is a common observation that multimeric enzymes tend to be less stable to freezing than their monomeric counterpart (17)
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. Mean-square displacements were extracted within the Gaussian approximation, and compared in a temperature range of 20-349 K
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. Conformational exchange is present when the difference between the R^sub 2^ rates measured with δ = 3
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. This agrees with the trend observed by CD and light scattering; for example, the heating curves recorded at 11 K/h show that apoA-1:DMPC disks undergo protein unfolding and lipoprotein fusion at higher temperatures than similar disks containing apoA-2 (Fig
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. Reconstitution of high-density lipoproteins
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