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. Analysis of the pH- and thermal-denaturation curves The fittings to Eqs
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. All fluorescence spectra were corrected for background scattering as measured with pure buffer
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. 1 M potassium cyanide and 1 mM EDTA in 0
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. 15 M Tris-HCl, pH 8, followed by column chromatography (23)
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. On repeating the process we found a consistent decrease in fluorescence intensity at 300 nm accompanied by an emerging red component in the spectrum and enhanced scattering from the solution, the latter response revealing partial unfolding of the macromolecule with exposure of buried W48 to the solvent and concomitant formation of protein aggregates
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. From the modeling results, it is found that wettability can be improved with larger substrate perimeters and smaller immersion depths, because the perimeter of the substrate (P) increases the total wetting force and the smaller immersion depth decreases the buoyancy force and increases the wetting force
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. Stress-strain curves of adsorbed protein layers at the air/water interface measured with surface shear rheology
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. This indicates that the Bis-ANS molecule is too big to completely fit in the thyroxine binding cavity and likely there is only room for one-half of the symmetric molecule
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. CONCLUDING REMARKSGiven the severity of many diseases associated with protein misfolding and aggregation, including the amyloidoses and the transmissible spongiform encephalopathies, research in this area is intense
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. , 2003) of the native structure before protein aggregation have begun to emerge
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. The multistep precipitation process was demonstrated in a laboratory experiment on the growth of submicron ankerite crystals, overgrown by submicron siderite and pyrite crystals, overgrown by subrnicron magnesite crystals, overgrown by subrnicron siderite and pyrite
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. Our results show that dimeric EI^sup sc^ acquired a folded conformation at physiological pH: however, 8-anilino-1-naphthalene-sulfonic acid (ANS)-binding experiments, FTIR, and CD measurements suggested that the protein populated a molten-globule-like species at low pH
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. Spectro/Por dialysis tubing, molecular mass cutoff 8 kDa, was a regenerated cellulose obtained from Spectrum Laboratories, NBS Biologicals (Huntingdon, UK)
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. The amount of aggregated material, or the composition of the coexisting phases, was measured by centrifuging the samples for 5 min at 4000 g, removal of the upper phase, and determination of protein content of the aggregates or the concentration in each coexisting phase using spectrophotometry
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.Literature CitedAigner, P
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