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. However, when the concentration of GdnHCl in the sample was raised to 2
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. This value (311 ± 5 K, at 4 µM protein concentration, 315 ± 9 K, at 6 µM protein concentration) was similar, within the experimental uncertainty, to that obtained in the intact EI^sup ec^ (4), where two tryptophan residues are located at the C-terminal region of the protein
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. The Θ^sub 222^(t) data show a rapid signal loss at any Gdm HCl concentration, indicating fast unfolding within the dead-time of the experiment (30 s), which precludes its quantitative analysis; the amplitude of this rapid phase increases with increasing denaturant concentration (Fig
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. At 1 M GdnHCl, one thiol group of GR became accessible to modification with 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB), whereas no changes could be detected in the spectroscopic properties (fluorescence, circular dichroism) of the protein
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. The DSC curves were obtained using a scanning rate of 0
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. That this is indeed the case is shown by comparing cavity statistics in the EV limit to values obtained for freely rotating chains
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. Light scattering To estimate protein aggregation occurring upon thermal unfolding, the RF-1501 Fluorospectrometer was employed to measure elastic scattering at 500 nm
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.A schematic representation of the TTR misfolding reaction from the unfolded state through the A-state is presented in Fig
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. Before studying the association of membrane-bound proteins, it was necessary to determine whether pressure affects the properties of the membranes themselves and whether pressure will affect the membrane association of peripherally bound proteins
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