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. 4 shows quenching of GR fluorescence by iodide
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. Biologists today endorse two principal but competing hypotheses to account for the evolution of degenerated eyes
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. The I [arrow right] A transition, which was referred to as the dominant unfolding process above, began at around 70°C and the midpoint of the transition (T^sub 1/2^) occurred at ~84°C (see Table 1)
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. Prophase I-arrested stage VI X
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. For example, the calculated ratio of k^sub cat^ (DmAChE/HuBChE) for hydrolysis of various substrates at 25°C is 1
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. The covariance matrix and thus the configurational entropy were calculated for three different sets of atoms (see Table 1):1
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. In contrast, the SL 4WJ shows a dramatic shift from very low E (0
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. Tertiary structure formation in the hairpin ribozyme monitored by fluorescence resonance energy transfer
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. Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures
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. It was difficult to distinguish in this research whether the pretransitional changes occurred in the catalytic domain or the dimerization domain of ACYI
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. This article deals with the unfolding reactions of the nonglycosylated recombinant form of the protein rSBA and its comparison with the glycosylated counterpart gSBA
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. First, we present a detailed description of the methods used in our work
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.A sample of ~200 mg DmAChE was dialyzed against, and then lyophilized from 0
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. We also employed the classic and widely used, although poorly understood, amyloid fluorescence dye ThT, which is known to interact with amyloid fibrils (Naiki et al
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. The difference in configurational entropy between two molecular conformations a and b can be estimated as ΔS = k^sub B^/2 ln(detσ^sub a^/detσ^sub b^), where σ^sub a^ and σ^sub b^, are the covariance matrices of atomic positional fluctuations of the two conformers and k^sub B^ is Boltzmann's constant
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.THERMAL ANALYSISWe determined the crystalline melting temperatures T^sub m^, of the IPP, EVA 9%, and EVA 19% polymers by DSC as 165, 83, and 97°C, respectively
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.)20Japan Sea-FarmingEarly 23 Apr '97Associationand late(Miyazu, Kyoto Pref
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. This feature of FRET has been used extensively to study the structure and dynamics of biological molecules and assemblies, in ensemble as well as more recent single-molecule measurements (Selvin 2000; Klostermeier and Millar, 2001b; Weiss, 2000; Ha, 2001)
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