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. Then it was subjected to ammonium sulfate fractional ion of 30%
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. Although the average shape in the EV limit is that of a prolate ellipsoid, the fluctuations in the R^sub g^ and δ-values are large
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. For PPA, which already exposes tryptophan residues to the protein surface in the folded state, a blue shift of the emission spectrum occurs upon thermal unfolding (see Fig
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. The dynamics is similar to that found for the "wraparound" binding mode in some aspects, e
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. 2 and in Supplementary Material (Table S1)
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. In this study we sought to resolve this contradiction and emphasize the phenomenological distinction between DNA compaction and DNA binding processes in the case of DNA compaction by monocations
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. Formation of an amyloid reflects an alternative to the native packing conformational struggle of a polypeptide chain to 1), reduce its surface accessible area; 2), saturate hydrogen bonding; and 3), reach an alternative non-native global free energy minimum (Gazit, 2002)
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. To estimate errors, several independent simulations could be performed and compared
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. 2 VitellogenesisOvary firm and yellow or Oogonia and developingoccasionally cream inoocytes still visible, butcolor
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. The inability of two- and three-way junction ribozymes to fully populate a docked conformation reveals the importance of correct helical junction geometry as well as loop elements for effective ribozyme folding
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. These distances are in agreement with the corresponding values obtained from the present spFRET experiments (see Results section above)
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. (11), we obtained a scaling exponent of v = 0
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. In addition, other properties of the unfolded states, most probable structural compactness or simply differences in the conformational scrambling, also affect the magnitude of red shifts
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