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Dissecting the Pretransitional Conformational Changes in Aminoacylase I Thermal DenaturationSu, Jing-TanABSTRACT Aminoacylase I (ACYI) catalyzes the stereospecific hydrolysis of L-acylamino acids and is generally assumed to be involved in the final step of the degradation of intracellular N-acetylated proteins
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4 A) was the apparent peak consisting of the changes of most native bands in the amide I' region, whereas the peak shift from 66°C to 80°C resulted from the transition from pretransitional to the dominant structural changes
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To assay conformational preferences of highly denatured proteins, we quantify a variety of properties for EV-limit ensembles of 23 two-state proteins
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The preceding discussion suggests that harshly denaturing (as opposed to mildly denaturing) conditions can be thought of as close mimics of "perfect", rather than just good, solvents (50)
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For n [arrow right] ∞, the formula in Eq
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Van de Kamp, M
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Typically used AFM-probes respectively were Silicon SPM Sensors NCHR (force constant = 42 N/m; length = 125 µm; resonance frequency [asymptotically =]300 kHz; nominal tip radius of curvature ≤5 nm) from Nanosensors (Neuchatel, Switzerland)
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It is clearer in experiment than the subsequent dimensional reductions because it is the only one that makes significant changes to the radius of gyration of the protein
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Preserved larvae were measured to the nearest 0
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Since the T^sub m^ of IPP resin was 165°C, the curing temperature was limited to 200°C
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To further evaluate the contribution of solvent-induced shifts, we examined a model duplex DNA molecule whose structure is not expected to change significantly with Mg^sup 2+^ concentration changes in the 0
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