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. This point, however, seems contradictory with long-standing evidence that men have been the focus of the majority of medical research (often to the detriment of women), and there is little evidence that reliability of participants in contraceptive research is any different than in any other areas of medicine
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. 0 adjusted using DCI or NaOD
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. For example, the HIV 1 gp-120 protein fails to reach its final folded stale in the absence of glycosylation, whereas the nonglycosylated RNase A has lower folding rates compared to its glycosylated counterpart, RNasc B (10-12)
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. Theory predicts that ensemble-averaged internal distances will scale like ensemble-averaged end-to-end distances such that [left angle bracket]R^sup 2^^sub ij^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|) = [left angle bracket](r^sub i^ - r^sub j^)^sup 2^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|)~1 (39)
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. Here, [left angle bracket]R^sup 2^^sub e^[right angle bracket] is the mean-squared end-to-end distance, R^sup 2^^sub ij^ = |r^sub i^ - r^sub j^|^sup 2^, and we choose r^sub i^ and r^sub j^ to be the position vectors of α-carbon atoms of residues i and j, respectively
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. Protein concentration was 4 µM
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. A measure of conformational entropy change during thermal protein unfolding using neutron spectroscopy
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. The interaction neither belongs to the category of lock-and-key nor to that of induced-fit
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. Repetitive heating and cooling of the same apoA-2:DMPC sample at 1
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. We therefore used the low molecular weight IPP polymer (PP3825, 22 µm particle size) in the subsequent tests
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. 23 DNA from calf thymus (Sigma) was used to prepare a standard curve
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. This paper is dedicated to the memory of our colleague Bernie O'Brien (1959-2004)
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. This indicates that subtle conformational changes are induced by low concentrations of GdnHCl (The existence of partially folded intermediate states in the unfolding of GR is indicated by a comparison of fluorescence spectral shift, anisotropy, iodide quenching, and CD data as a function of GdnHCl concentration
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