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The presence of PEP and Mg^sup 2+^ did not change substantially the secondary structure of the protein, as indicated by FTIR measurements calculator house loan .
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Fluorescence spectroscopy Intrinsic fluorescence was measured using a RF-1501 rluorospectrometer (Shimadzu, Duisburg, Germany) and a LS55 luminescence spectrometer (Perkin-Elmer, Rodgau, Germany) calculator house loan .
This trend is consistent with a pronounced binding capacity of oligomers whose subunits tend to dissociate in frozen aqueous media thereby making accessible large hydrophobic patches of the subunit interface calculator house loan .
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MethodsSamples for fluorescent microscopy were illuminated with 365 nm ultraviolet light, and fluorescence images of DNA molecules were observed using a Zeiss Axiovert 135 TV microscope equipped with a 100× oil-immersed lens and recorded on S-VHS videotape through a Hamamatsu SIT TV camera calculator house loan .
Before initiation of the aggregation reaction, TTR is in the form of unfolded monomers calculator house loan .
We compared the properties of DCVJ with the known amyloid specific dye ThT and found striking similarities, including fluorescence lifetimes and viscosity dependence on fluorescence intensity calculator house loan .
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