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. Crystal structure of D-aminoacylase from Alcaligenes faecalis DA1
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. The obtained peak maxima around 336-338 nm indicate rather small red shifts (~2-3 nm) with respect to the native state (see also Fitter and Haber-Pohlmeier (14))
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. Stimulated by the biological importance of this subject, many in vitro investigations of DNA interaction with K+ and Na+ ions as well as other monovalent alkali cations (Li+, Rb+, and Cs+) have been performed by biophysicists (Bleam et al
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. pH-independent binding and fluorescence of DCVJOne problem frequently encountered with ThT is the pH-dependent fluorescence in amyloid fibril formation measurements
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. The high-temperature transition in apoA-2: DMPC disks (solid circles, Fig
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. This is not yet reachable for proteins with the current computation timescale of nanoseconds
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. Indeed, the significant stability of GR dimers is illustrated by the fact that no changes in fluorescence emission of this protein take place even in the presence of 2 M urea and at a hydrostatic pressure of 3
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. Low temperature induced conformational changes of aminoacylase
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. DCVJ was found to be very sensitive to formation of early oligomers and showed the strongest apparent binding affinity of the probes in the study
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.The melting and kinetic CD data in Figs
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