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Interestingly, the results from fluorescence and infrared (IR) spectroscopy clearly indicated that pretransitional conformational changes occurred at temperatures from 50°C to 66°C during ACYI thermal denaturation
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To avoid misleading results from the heavy overlapping of the original IR amide I' band, FSD data, which maintained the intensity of the original data, were used for the phase diagram analysis
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If folding were strictly driven by the formation of local contacts (100-102)
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Molten globule and protein folding
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(34)[FIGURE 3 OMITTED]Efferocytosis also increases the production of secretory leukoprotease inhibitor, which is an antiprotease with activity against elastase and cathepsin G
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However, upon unfolding transitions generally distinct shifts of the emission spectra are reported (see for example (4,9-14))
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(8) achieved magnetic resonance (MR) microscopic imaging of a large single cell, the Xenopus laevis oocyte whose diameter is ~1
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Instrumental aspects and data analysisNeutron scattering experiments were carried out on the backscattering spectrometer IN16 (energy resolution ΔE = 0
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A homodimeric peptide corresponding to the complete SEF2-1 basic sequence, henceforth called SEF2-1mp, was chosen as a good model system for NMR studies of the interactions between bHLH proteins and Ca^sup 2+^/CaM
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5, C and D) and shorter fluorescence lifetimes (data not shown) reveal Trp to ANS and Bis-ANS fluorescence resonance energy transfer both in the native and misfolded states, indicating that the Tips and the probes are in close proximity (Conrad and Brand, 1968)
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For Bis-ANS, the fluorescence in the presence of unfolded TTR is significant
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Although the data from such measurements are noisy due to the short data collection time (2 min), we found good correlations with the aggregation kinetics of ANS binding using steady-state fluorescence (Fig
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We have previously found that activated PLCβ-Gβγ complex can be rapidly deactivated by Gα(GDP) subunits without dissociation, which led to the suggestion that Gα(GDP) binds to PLCβ-Gβγ and perturbs the activating interaction without significantly affecting the PLCβ-Gβγ binding energy
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CPM-PLCβ is sensitive to binding by Gβγ-subunits giving an ~200 cm^sup -1^ shift in emission energy and an ~2-fold increase in intensity (G
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Hence, a quasi-docked conformation could be visited many times during the course of hairpin ribozyme folding, thereby increasing the probability of forming the key tertiary contacts required for stable docking of loops A and B
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Samples for transmission electron microscopy were fixed in 0
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