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Unfolding was monitored by a variety of techniques, including intrinsic fluorescence emission, anisotropy and iodide quenching measurements, far-ultraviolet circular dichroism and thiol reactivity measurements calculator loan site uk .
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Solvent-accessibility of aromatic residues (acrylamide quenching experiments) Quenching experiments in the presence of the effectors yielded quenching constants in the presence of Mg^sup 2+^ (2 calculator loan site uk .
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