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. Coupling the phosphotransferase system and the methyl-accepting chemotaxis protein-dependent chemotaxis signaling pathways of Esclierichia coli
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. Decomposition of protein tryptophan fluorescence spectra into log-normal components
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. The acquisition parameters were TR = 2000 ms, SW = 10 kHz, acquisition size = 4096, NEX = 256, T^sub acq^ = 8
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. Address reprint requests to Chaejoon Cheong, Korea Basic Science Institute, 52 Eoun-dong, Yusung-gu, Daejeon 305-333, Korea
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. 9 µeV, corresponding to a time window of ~730 ps; accessible Q-range: 0
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. Besides picosecond motions, all residues also experience internal motion with an effective correlation time of ~2
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. It should however be noted that Bis-ANS still fluoresces very strongly in the presence of large aggregates (Figs
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. Several amyloidogenic in vitro assays employ acidic pH for generation of amyloid fibrils; however, ThT shows very modest fluorescence at acidic pH when bound to protofibrillar amyloid of TTR (Fig
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. If this idea is correct, then the volume associated with Gβγ activation of PLCβ corresponds to the difference in volume changes for PLCβ-Gβγ alone and in the presence of Gα^sub s^(GDP), ranging from ΔV ~3 to 15 ml/mol (Table 1)
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. Structure of the high affinity complex of inositol trisphosphate with a phospholipase C pleckstrin homology domain
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. Since spectral shift measurements showed a sharp transition between 1 and 3 M GdnHCl (Fig
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. Here we applied this method to analyze delta and gamma dispersions for protein solutions
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. Pappu Department of Biomedical Engineering and Center for Computational Biology, Washington University in St
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. Experiments at pHAcrylamide quenching experiments, measured by excitation at either 280 or 295 nm, showed exponential Stern-Volmer plots (Table 1 ), which yielded similar results: the K^sub sv^ were larger at acidic than at neutral pH, and the experiments in GdmHCl had the largest values of K^sub sv^
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.2 M^sup -1^ and v = 0
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. Due to a tight sealing of the quartz cuvettes temperatures of protein solutions up to ~120°C were applicable without evaporation and boiling
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. In vitro mutagenesis and expression in Xenopus oocytes
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. Akke and co-workers (38) have also found larger ΔH^sup #^ values for residues that are part of regular secondary structure and smaller values for loop residues, in their study on the C-terminal domain of CaM
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.It is well accepted that water in the vicinity of the protein globule contributes toward its hydrodynamic behavior, particularly in dilute solution (65,66)
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.We thank Dagmar Klostermeier for assistance in preparing some of the hairpin ribozyme constructs
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