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. Hence, we can conclude that the intermediate obtained in the chaotrope-induced denaturation of rSBA is also monomeric in nature
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. Interestingly, similar results are obtained for the protein modeled as a fully extended conformation (Fig
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. 1 C) and the FTIR data acquired at pH 3 (Table 2)
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. These intensity profiles are reminiscent of binding curves except for assuming a slightly sigmoidal shape due to a more gradual increase in the signal in the low concentration range
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. Thanks to an unusually blue shifted Trp fluorescence spectrum of native azurin, a measure of the protein fraction remaining native after freezing-thawing can be obtained directly from the residual fluorescence intensity at 300 nm (λ^sub ex^ = 292 nm), a wavelength where denatured-aggregated proteins were found to contribute little to the overall intensity
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. The fluorescence profiles of all seven proteins in ice, at -13°C, are compared in Fig
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. The highly purified HuBChE was stored at 4°C after lyophilization from 10 mM sodium phosphate, pH 8
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. Stimulated by the biological importance of this subject, many in vitro investigations of DNA interaction with K+ and Na+ ions as well as other monovalent alkali cations (Li+, Rb+, and Cs+) have been performed by biophysicists (Bleam et al
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. It is recognized that the binding of the part of monocations to DNA can be specific in the minor or major grooves of B-form DNA (for review, see Hud and Polak 2001; McFail-Isom et al
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. 1, software for running the spectrometer and deconvolution and analysis of decays
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. ApoA-II/apoA-I molar ratio in the HDL particle influences phospholipid transfer protein-mediated HDL interconversion
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. BD=body depth; BL=body length;ED=eye diameter; HD=head depth; HL=head length; PAL=preanal length
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. Our results illustrate how these secondary structure elements combine to direct the tertiary folding pathway of the ribozyme
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