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. To summarize the above described observations one can state, that GndHCl-induced unfolding reveals for all α-amylases rather uniform emission spectra with large red shifts, whereas thermal unfolded states appear with more divers and in general with much smaller λ^sub max^-values as compared to the former
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. Similar increase in fluorescence quantum yield was evident in 50% w/w PEG (MW 3350; data not shown) as in 50% glycerol showing that increased viscosity is the main reason for the increased quantum yield
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. The dependence of the ANS fluorescence intensity, in ice at - 13°C, on the starting ANS concentration in solution (10-200 µM) is shown in Fig
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.INTRODUCTIONMany diseases are caused by protein misfolding
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