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. Lower concentration rates of protein to trypsin were used (100:1, 200:1, and 300:1), but in all these experiments the amount of nondigested EI^sup sc^ in the absence of effectors was insignificant after 1 min of reaction (data not shown)
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. In this pH range, the minimum at 208 nm disappeared, whereas that at 222 nm was still present
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. For the analysis of the relaxation data, the apparent overall tumbling time is an important parameter and we briefly describe the relevant equations
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. This so-called block average procedure has been applied, for example, to obtain standard errors of the average area per lipid molecule in membrane simulations (Anezo et al
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. Researchers have reported on polymer particle formation by phase separation [2, 9, 12, 16], but these works have not included the use and testing of the particles as pigment-loaded toner for xerography
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. Toner particle size is very important in xerographic printing for obtaining good optical resolution
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. In this method, the fluorescence of donor and acceptor are simultaneously recorded using avalanche-photodiodes as individual molecules traverse the excitation volume in a confocal microscope, and the FRET efficiencies from many such single-molecule observations are plotted in the form of a histogram
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. We also monitor liquidity ratios by payer class
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. Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein
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.Some phagocytic receptors interact directly with apoptotic cells; however, most interactions occur indirectly through bridging proteins (Fig 2)
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. The three cubes in the image indicate the actual position and width selected for the recording of localized spectra from isotropic voxels of (180 µm)^sup 3^ in each compartment
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.The authors thank Jacques Colletier for useful discussions on cholinesterase biochemistry as well as the IN16 instrumental team (Bernhard Frick, Thilo Seydel, and Matthias Elender) and the IN13-CRG team (Marc Bée, Francesca Natali, and Sebastien Vial) at the Institute Laue-Langevin, Grenoble, France
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. All measurements were performed at room temperature
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. The spectra obtained upon Trp excitation (290 nm) are shown in Fig
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. Bis-ANS shows a wide variety of fluorescence responses and fluoresces most strongly when bound to monomeric A-state TTR and surprisingly also fluoresces strongly when bound to unfolded monomeric TTR
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. Bis-ANS has a shorter lifetime than ANS but also displays two different components with a more even distribution
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. The reaction rate is even slower above 24°C, suggesting that the heat-induced disk-to-vesicle conversion is thermodynamically irreversible
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. One barrier to the use of xerography for printing textiles is the lack of textile-specific toners that produce the required textile properties
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. Three days after the final immunization, the spleens of the mice were removed and passed through a mesh (mesh size: 100 [micro]m)
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