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The glycan was interacting with the protein via 27 hydrogen bonds and 53 hydrophobic interactions (Fig
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Polymer principles in protein structure and stability
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For instance, the barnase (45) and oxalocrotonate tautomerase (46) bind specifically to their rigid target to form a specific complex with essentially one overall conformation
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Also plotted in Fig
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The nature of the colloidal 'glass' transition
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0) or 25 mM HCl (pH 1
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Bis-ANS shows a wide variety of fluorescence responses and fluoresces most strongly when bound to monomeric A-state TTR and surprisingly also fluoresces strongly when bound to unfolded monomeric TTR
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Pressure has profound effects on the highly compressible membrane interior promoting phase transition to gel phases and, for the studies presented here, the phase transition will occur ~1
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The amino acid sequences of these proteins are comprised of tandem 11/22-residue repeats that have high propensity to form amphipathic α-helices with distinct charge distribution and large apolar faces that bind lipid (Segrest et al
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1973, Errington 1930, Lowe 1980, Raczynski and Ruprecht 1974), most have not (e
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The liposomes eventually rapture, releasing the drug
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"McClellan said the agency would release a revised definition of AMP later this year for public comment, along with an initial round of AMP data, based on the new definition
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. Conversely, at acidic pH, the percentage of β-turns appearing at 1668 cm^sup -1^ was decreased, and the percentages of helical structure, loops, and disordered structures (bands at 1648 and 1657 cm^sup -1^, which could not be independently assigned at pH 3) were higher (50
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