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Ice-induced ANS binding to other proteins To examine whether the ice-induced perturbation observed for azurin is common to proteins in general, ANS binding experiments were extended to a set of seven other proteins including monomeric BSA, α-amylase and β-gal, dimeric LADH and tetrameric ALD, YADH, and LDH canadian bank loan rate .
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For diffusion spFRET measurements, the resulting ribozyme complexes were diluted with annealing buffer (missing NaCl and supplemented with 0 canadian bank loan rate .
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