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. Protein structure is strongly related to protein-solvent interactions and hydration state
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. The Kratky profiles reveal the presence of three distinct regimes for each sequence
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. Kratky profiles for proteins in the EV limit were compared to those of folded proteins and ideal, freely rotating chains (43)
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. coelicolor, EI^sup sc^, indicate that its thermal denaturation behavior is different from that of other EI proteins, since only one transition is observed (23)
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. An excitation wavelength of 280 nm (bandwidth 3 nm for the Perkin-Elmer and 10 nm for the Shimadzu spectrometer) was applied while emission spectra were recorded in the range between 300-450 nm (bandwidth 5 nm and 10 nm for the Perkin-Elmer and the Shimadzu spectrometer, respectively)
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. Data analysisAll spectra were analyzed using SYBYL software (TRIPOS)
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. Similar microscopic measurements and analysis were performed in solutions of PEG with a higher molecular weight and the same PEG concentration as used in the above experiments
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