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. ^sup 15^N-{^sup 1^H} NOE values were determined from the ratios of peak volumes recorded in presence and absence of proton saturation
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. Scarlata, unpublished), allowing us to measure the apparent K^sub d^ at a given membrane concentration (Fig
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. Fluorescence experiments at pH 7 and 9 showed a sigmoidal transition with T^sub m^ = 311 ± 4 K (Fig
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., K^sub p^ ~ 70 as opposed to 10 µM; Philip and Scarlata, 2004)
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.33 K/min (80 K/h) yields similar CD melting curves (not shown), with the apparent T^sub m^ = 84°C identical to that of intact disks
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. However, the herein-observed morphological variation after increasing temperature bears a more profound meaning in light of the ongoing debate as to the identity of the protein's aggregation-prone conformation (completely unfolded, or partly unfolded protein) and our own study, showing that under similar conditions, bovine insulin is substantially more unfolded at 70 than at 60°C (Dzwolak et al, 2003)
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.6) n=20HL/BLx100Preflexion21
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.The average burst duration for the SL 4WJ at 10mM Mg^sup 2+^ was estimated from two-channel data acquired with time bins of 50 µs, by averaging the burst durations within each 500-µs rebinned point lying above a threshold of 40
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