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Dissecting the Pretransitional Conformational Changes in Aminoacylase I Thermal DenaturationSu, Jing-TanABSTRACT Aminoacylase I (ACYI) catalyzes the stereospecific hydrolysis of L-acylamino acids and is generally assumed to be involved in the final step of the degradation of intracellular N-acetylated proteins
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The fully deuterated samples were obtained by incubating the protein solutions in a water bath at 50°C for 15 min, and then the samples were cooled to room temperature and lyophilized
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by using theoretical and experimental approaches
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Encapsulation techniques, either using liposomes (29) or sol-gel glasses (30,31), have recently been used to trap individual proteins in small molecular voids
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The previous NMR data were consistent with two schematic models of the CaM dimer
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This idea is based on studies suggesting that multiple Gβ peptides can bind to PLCβ and give different levels of activation (Buck et al
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The unfolding was triggered at t = 0 by a temperature-jump from 25°C to 75-95°C and was monitored by CD at 222 nm (Fig
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These elements are particularly vulnerable to deterioration due to the heat and shear applied during processing
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On average, these fibrils are -80-500 nm long, and rather uniformly 4
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Reinterpretation of GCN4-pl folding kinetics: partial helix formation precedes dimerisation in coiled coil folding
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We have also tested equilibrium constants of 1
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Whereas probabilistic theories do not ascribe a role to players' cognitive attributions, there were systematic differences in players' attributions regarding the causes of the outcomes of play
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