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. Size exclusion chromatography was used to investigate the quaternary structure of GR (Fig
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. The measured hydration number of U^sub A^ was close to that of the accessible water number (1340) of a protein molecule calculated for a fully extended structure, indicating that the structure of U^sub A^ is extended but somewhat more compact than that of a fully extended state
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. Furthermore, in some cases the differences in intensity signals from folded and unfolded states are very small, which makes precise measurements difficult (12)
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