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. The unite probability of linding large cavities within the interior of denatured proteins emphasizes two points: First, the volume occupied by a chain is filled inefficiently when compared to either a folded protein or a freely rotating chain
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. The average topology in the EV limit is independent of amino acid sequence
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. Steady-state ANS binding ANS binding was detected by collecting fluorescence spectra at different pH in the presence of 100 µM of dye
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. The occurrence of such states depends upon the range and strength of the attractive and repulsive forces acting between the particles
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. This medium is designated DMEM/ITS+/TGF-[beta]1 hereafter
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.5 kbar (data not shown), a pressure at which a large number of protein dimers and higher oligomers have been found to undergo subunit dissociation (Gross and Jaenicke, 1994; Heremans and Smeller, 1998)
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. This suggests that the association of hydrophobic moieties might follow the disruption of secondary structures from N to A states
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. The activity decreased rapidly at higher temperatures (>65°C) due to inactivation of the enzyme
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. Folding mechanism of the CH2 antibody domain
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.75 ml g^sup -1^ was then used to obtain the mass of water in the hydrodynamic domain of the protein, and from this the volume fraction of protein at known mass fractions was estimated
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. Similar microscopic measurements and analysis were performed in solutions of PEG with a higher molecular weight and the same PEG concentration as used in the above experiments
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. This is followed by the binding of the chemokine co-receptor to gp120 (Berger et al
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