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In a previous study from the laboratory, we reported that the monomer of gSBA has a hydrodynamic radius of ~3
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Generation of conformational ensembles We have adapted conventional Markov-chain Metropolis Monte Carlo simulation strategies (60,61) to generate equilibrium ensembles for each of the protein sequences in the EV limit
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In the EI protein from Salmonella typhimurinm
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It is obvious that this also can have different effects on the microenvironment of tryptophan residues
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The second objective was addressed by monitoring the ANS binding capacity of a protein, azurin, mutated to alter its stability and internal dynamics through the creation of internal cavities and the elimination of a disulfide linkage
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2 should not be considered a binding curve, it may still be instructive to estimate an apparent average affinity of ANS for the four azurin species
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The Sn-Pb solder experiences an increase in maximum wetting force when the solder bath temperature is increased from 255°C to 275°C, whereas 295°C decreases the wettability
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However, this same aspect leads to a lower affinity and specificity than for (cooperative) rigid binding (with all target sites simultaneously and rigidly interacting)
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Indeed, compaction potential appears to be inversely related to the binding activity of monocations
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Prefibrillar amyloidogenic oligomers and protofibrils of misfolded TTR were generated in vitro through induction of the molten globule type A-state from acid unfolded TTR through the addition of NaCl
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The aggregation reaction of monomeric acid unfolded TTR (14 µM) was initiated by addition of 25 µl 4 M NaCl to a 1000 µl sample, giving a final NaCl concentration of 100 mM (23°C)
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Don't they see that it's the one place it should not be? It's not just in the town but in the harbour, which means it will spoil the view that Turner made," he said
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The α-helix content was estimated from the value of [Θ] at 222 nm (Mao and Wallace, 1984)
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Interestingly, the light-scattering melting curves show a smaller heat-induced increase in the particle size for apoA-1 than apoA-2-containing disks (Fig
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In Proceedings of a Meeting Held at Allerton House
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4 mm BL preflexion larvae
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2] temperatureof sea(at 10 mCruise surface)depth) Depth (m)SM-IX7
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Cheese also is rich in branched chain amino acids (BCAA), known to play a role in insulin regulation and glucose sparing
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Each 8-ns trajectory was divided into 16, 8, and 4 blocks of non-overlapping trajectory segments of 0
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Stuck between the interests of pharmaceutical companies and insurers, the FDA did nothing for four years
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Preying on the elderly
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He can't believe that the casinos destroyed by Hurricane Katrina may qualify for millions of dollars in tax breaks as part of the Bush administration's plan to spur redevelopment on the Gulf Coast
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If you stand and still lose, you can blame it on the dealer or attribute it to the dealer's luck because you obviously cannot control what the dealer draws
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This finding is in line with Walker's (1988) assertion that blackjack players disregard long-term probabilities of winning or losing, but alter their betting behavior according to short-term fluctuations of outcome
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