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The stability of GR dimers is also largely generated by the interface domain, since double or even single mutations in this region are sufficient to lead to the assembly of less stable dimers in the E
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This is referred to as U'
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3%)), but similar, within the error, to those observed at pH 10 (55%)
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Proteins of the phosphoenolpyruvate:sugar phosphotransferase system in Streptomyces: possible involvement in the regulation of antibiotic production
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For most proteins, which typically bear more than one tryptophan, the observed emission spectrum is composed of multiple contributions of the five spectral classes
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Study of the "molten globule" intermediate state in protein folding by a hydrophobic fluorescent probe
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Since soman inhibition is known to stabilize the folded state, this implies that the soman-inhibited unfolded state
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Experimental and modeling results are clarified as measured and estimated, respectively
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lib and b^sup /^) than for 255°C (Fig
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ACKNOWLEDGEMENTSThe authors acknowledge the financial support provided by the Research Grant Council of Hong Kong for the project "Wetting Kinetics and Interfacial Interaction Behavior between Lead-Free Solders and Electroless Nickel Metallizations," CERG Project No
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It is insensitive to small variations in ^sup 15^N chemical shift anisotropy (CSA) (from -150 to -200 ppm) (22)
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Typically used AFM-probes respectively were Silicon SPM Sensors NCHR (force constant = 42 N/m; length = 125 µm; resonance frequency [asymptotically =]300 kHz; nominal tip radius of curvature ≤5 nm) from Nanosensors (Neuchatel, Switzerland)
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For a molecule existing in two different states but with similar atom-positional fluctuations, similar configurational entropies are expected for the two states
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Several are located in the putative trimerization interface, S243, C378, and 1439, and two of them, V255 and L260, are located in the β-turn that connects the inner and outer domains of gp120
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jpYosuke Onishi Tomoii Ikeda Hironobu Komaki Kansai Environmental Engineer Center 1-3-5 Azuchi-cho, Chuo-ku Osaka City, 541-0052 Osaka, JapanMuneo Okiyama Ocean Research Institute University of Tokyo 1-15-1 Minamidai, Nakano-ku, Tokyo, Japan
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This work was supported by grants from the National Institutes of Health to D
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British Museum (Natural History), London, UK
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055 -2,617 30Average annual effect-15,517
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. To test whether there is a relationship between the extent of ANS binding induced by freezing and the instability of azurin to the freeze-thawing process, we have determined the degree of irreversible denaturation of WT and mutant species when subjected to repeated freeze-thawing cycles
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