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The binding affinity was compared between the different probes defining an intensity 50 value, I^sub 50^, being the half height of the maximum emission intensity, corresponding to an apparent dissociation constant car georgia in loan title .
In contrast to the large effects of pressure on the membrane interior, pressure does not appear to affect the membrane surface charge and has little effect on surface hydration (Rebecchi et al car georgia in loan title .
13 kJ K^sup -1^ mol^sup -1^; see Fig car georgia in loan title .
We have also tested equilibrium constants of 1 car georgia in loan title .
A 96 well plate was used and 40 µl of both the standards and samples were pipetted into each well; 250 µl of DMB solution was added to each and the absorbance at 595 nm was determined using a microplate reader (Bio-Rad Ltd, Hemel Hempstead, UK) car georgia in loan title .
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