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For lipid T^sub 2^ measurements in the cytoplasm, localized spectroscopy with (200 µm)^sup 3^ voxels was employed with 10 different echo times in intervals of 4 ms, starting at 6
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The presence of exchange between multiple-target sites and flexibility in the bound state leads to an important advantage over rigid binding, namely that the affinity (and specificity) is finely tuned
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0 mM acetate buffer containing 1 M NaCl at 20°C
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. At 80°C, ACYI contains ~10% Class I, 60% Class II, and 30% Class III fluorophores, which suggested that most Trp residues in the protein were accessible by solvent
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. ApoA-2 has particularly large apolar helical faces (50% of the surface as compared to ~30% in apoA-1 and apoC-1) which, along with higher hydrophobicity of these faces, is thought to account for stronger association of apoA-2 with HDL (Brasseur et al
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. The smallest particle size (22 µm) occurred with a 10 wt% solution of PP3825 at 130°C dissolving temperature, so we used these conditions for the subsequent tests
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. In the case of the SL 4WJ, for the broad/asymmetric peaks at some of the high Mg^sup 2+^ concentrations, these fitted Epeak values are representative of the peak positions, but are higher than the average FRET efficiencies by ~0
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