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To assay conformational preferences of highly denatured proteins, we quantify a variety of properties for EV-limit ensembles of 23 two-state proteins
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Our studies have demonstrated that high airway elastase activity in CF patients suppresses efferocytosis by acting on both the phagocyte and the apoptotic cell
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In some cases (when using the Shimadzu spectrometer) an UGl filter (Schott
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1 ppm, and the aceturate peaks (a, d, and e) at 1
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Instrumental aspects and data analysisNeutron scattering experiments were carried out on the backscattering spectrometer IN16 (energy resolution ΔE = 0
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Nonlinear temperature dependence of the crystal structure of lysozyme: correlation between coordinate shifts and thermal factors
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Thus, the N-H^sup N^ bond vectors within an α-helix must have similar (τ^sup o^^sub m^)^sub app^, and the average (τ^sup o^^sub m^)^sub app^ for each helix can be taken to reduce the error
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Supercoiling in closed circular DNA: dependence upon ion type and concentration
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Using the hairpin ribozyme as a model system, we specifically address a key question in RNA folding-how do preformed secondary structure elements dictate the course of subsequent tertiary folding events? Currently, little is known about how secondary structural elements affect the ribozyme conformational distributions important in the course of folding
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--Biomedical SciencesUniversity of California at San Francisco School of Pharmacy MS
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. Although gSBA was seen to demonstrate very high conformational stability, rSBA exhibits comparatively much lower firmness to resist the structural perturbation induced by the denaturant guanidinium hydrochloride
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. In C3A/C26A the elimination of the superficial C3-C26 disulfide bridge, linking the ends of strands β land β2, has little effect on the compactness of the protein core and, judging from the phosphorescence properties of W48 embedded in it (18), internal motions remain restrained
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. Since soman inhibition is known to stabilize the folded state, this implies that the soman-inhibited unfolded state
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