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. According to this view, the tight and specific association of subunits in the native GR dimer may be facilitated by contacts between MG monomers
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. Second, unlike hard-sphere potentials, which stipulate that all sterically allowed conformations are isoenergetic, soft-core potentials encode the requisite conformational specificity (48,53,58)
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. As already mentioned above, there is indication from previous studies on several α-amylases that a structural unfolding is followed by an irreversible process (14,22-25)
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. We examined two single point cavity-forming mutants, I7S and F110S, whose internal cavities of 40 and 100 [Angstrom], respectively, appear to be fully hydrated (18), and the double mutant C3A/C26A in which the disulfide bridge linking strands β1 and β2 has been severed
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. Potential bias in NMR relaxation data introduced by peak intensity analysis and curve fitting methods
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. Osmotic stress for the direct measurement of intermolecular forces
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. The results presented in this report describe the misfolding, oligomerization, and aggregation process of TTR that was induced from unfolded TTR through induction of the molten globule A-state
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. The Θ^sub 222^(t) data show slow unfolding on a timescale of 10^sup 2^-10^sup 3^ s (as compared to 10^sup -8^-10^sup -10^ s for the helix-to-coil transition in solution; Yang and Gruebele, 2003), implicating high free-energy barrier ΔG*
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. It may be therefore hypothesized that the protofilament structure gives rise to an inherent mechanical strain, which may be relaxed either by a collective twisting of associated protofilaments, or curling them
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. It is worth noting that a multi-event equilibrium unfolding/ dissociation of human GR by GdnHCl has been previously reported (Nordhoff et al
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. 4 shows the variation of the hydrodynamic radii of the protein at different denaturant concentrations
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. That the peptide remained dimeric was also confirmed by SDS-PAGE
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.1 are all in nonhelical regions except residues 72, 73, and 75, which are pail of the less well-defined C-terminal part of helix IV
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