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MATERIALS AND METHODS Materials Horse apomyoglobin was prepared by using Hapner's method (Hapner et al
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GdnCl-induced denaturation of rSBA and gSBA Chaotropic equilibrium denaturation profiles in which the concentration of GdnCl was increased from 0 M to 7
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Until 2 M denaturant concentration
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However, it has been shown in various cases that (and how) under specific conditions λ^sub max^-signals can be used for quantitative analyses of unfolding transitions (4,12,15)
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Furthermore, since transcription levels will remain low until the midblastula stage when development speeds up, the cytoplasm is stuffed with high amounts of maternal RNA (40)
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A more likely relationship may be of a kinetic nature, comprising barrier-crossing and activation processes that are of a statistical nature
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Part of this uncertainty arises as a result of the polyampholyte characteristics of the protein
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At the lower concentrations, Newtonian behavior was observed at shear rates, γ, in the range 10-100 s^sup -1^
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Time-resolved fluorescenceTime-resolved measurements of tryptophan (Trp) fluorescence were recorded using an IBH (Glasgow, UK) 5000 U fluorescence lifetime spectrometer system with 1 nm resolved excitation and emission monochromators (5000 M)
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In general, the application of hydrostatic pressure in the range of 1-2000 bar (where 1 bar = 1
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The α-helix content was estimated from the value of [Θ] at 222 nm (Mao and Wallace, 1984)
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To assess the high- and low-molecular-weight products of the thermal disruption and reconstitution of the apoA-2: DMPC disks, we used negative staining electron microscopy and non-denaturing gel electrophoresis (Fig
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7 a is a pair of such units
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Relative head length remained constant, whereas relative head depth diminished during development (Fig
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5; and the bridging sheet (code: bridge) and the V3 loop (code: V3) of gp120; see Fig
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) 4 Pagrus major Fukui Prefectural SeaLate2 Jun '95Farming Center(Obama, Fukui Pref
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Success rates for ACI range from 60% to 90% depending on site and location of the cartilage defect
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, hit or sit) as a function of the player's total and the dealer's face card, according to the Basic strategy
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Although such behavior can be observed in real gambling, it was not observed in the present simulated gambling experiment
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