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The optimal temperature for ACYI activity is ~60°C (25,26), which indicates that it can be regarded as a thermostable enzyme
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Pathways of protein folding
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Our results show that dimeric EI^sup sc^ acquired a folded conformation at physiological pH: however, 8-anilino-1-naphthalene-sulfonic acid (ANS)-binding experiments, FTIR, and CD measurements suggested that the protein populated a molten-globule-like species at low pH
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and acquired at pH 3
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3 c reveals that the native state exhibits no affinity for aggregation which is also true for all other α-amylases within limits of protein concentrations used here
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2 and then adding the probe at a 2:1 molar ratio
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Differential association of the pleckstrin homology domains of phospholipases C-β1, C-β2 and C-Δ1 with lipid bilayers and the βγ subunits of heterotrimeric G-proteins
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1 C was rapidly cooled to 5°C and scanned again from 5 to 100°C
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This, as well as visualization of early unstructured aggregates, are the particularly advantageous features of AFM in studies on amyloidogenesis
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For example, milling is a surface operation, and it requires a minimum amount of lubricity
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Entropy calculations on a reversibly folding peptide: changes in solute free energy cannot explain folding behavior
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Relative fecundity (based on fertilized eggs) increased with age from 374 eggs/g for fish age 6 to 549 eggs/g for fish age 16
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1] = regression coefficients for the intercept and fork length, respectively
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In 2004, nearly 15 million Americans ages 12 and up--that's 6
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