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. Column calibration was done with a set of eight proteins of known molecular weights (thyroglobulin, 669 kDa; ferritin; 440 kDa; alcohol dehydrogenase, 150 kDa; bovine serum albumin, 66 kDa; ovalbumin, 45 kDa; carbonic anhydrase, 29 kDa; trypsinogen, 24 kDa; and cytochrome C, 12 kDa)
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. This equivalence in average topologies of denatured states explains why it has been reasonable to ignore the denatured state when assessing the contribution of topology to folding rates for small two-state proteins
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