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. Hence, not many orientations of the glycan chain upon attachment to the protein are possible and we assume that upon energy minimization, the structure obtained mimics the real situation
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. Data analysisAll spectra were analyzed using SYBYL software (TRIPOS)
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. A figure presenting the matrix-assisted laser desorption ionization mass spectra of the glycosylated and nonglycosylated form is shown in the Supplementary Material
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. 1 resembles the traditional hardsphere potential (57)
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. It is tempting to suggest, based on our results, that the species populated at high pH is also a molten-globule-like species, although it did not bind ANS (Fig
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. The observed red shift is caused by the fact that tryptophan residues are replaced from the less-polar interior of the protein to solvent exposed regions upon unfolding
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