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The sequence of the events was characterized by analyzing the signs of the peaks in the 2D IR correlation plots using rules proposed by Noda (29)
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The other components remained at the same level as at 30°C in the temperature range of 50-66°C
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Ice-induced ANS binding to other proteins To examine whether the ice-induced perturbation observed for azurin is common to proteins in general, ANS binding experiments were extended to a set of seven other proteins including monomeric BSA, α-amylase and β-gal, dimeric LADH and tetrameric ALD, YADH, and LDH
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Besides, the lack of lipid signal in the nucleus is consistent with the lack of yolk platelets in the nucleus (31,32) and with a previous lipid ^sup 1^H MRI study of the Xenopus oocyte (12)
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GENERAL DISCUSSIONThe compositions at which the relative viscosity (Fig
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Osmotic pressure and viscoelastic shear moduli of concentrated emulsions
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Time-resolved fluorescence anisotropy decay of ANS was shown to be a useful tool for giving further structural and kinetic information of the oligomeric aggregates
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The shortest incubation at 60°C (or 70°C) applied to insulin samples was only 30 s; yet as Fig
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After removing the PBS, 200 [micro]L of the hybridoma culture supernatant solution was added to the wells and incubated at room temperature for 30 minutes
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19Table 2Fish eggs used in the experimentEggStage of Samplingno
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Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Forster distance dependence and subpopulations
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