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from the University of Pennsylvania
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Spectra were averaged over three scans for each sample containing 3 µM GR in 30 mM HEPES, at pH 7
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Representative isothermal and thermal denaturation profiles show that glycosylation accounts for a stabilization of ~9 kcal/mol of the tetramer, whereas the difference in T^sub m^ between the two forms is 26°C
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These features (namely, the absence of tertiary structure, the lack of a sigmoidal behavior in thermal heating, solvent-exposure of hydrophobic residues, and a large amount of secondary structure) are characteristic of the so-called molten-globule species (47,48)
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Encapsulation techniques, either using liposomes (29) or sol-gel glasses (30,31), have recently been used to trap individual proteins in small molecular voids
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Here, the average (τ^sup o^^sub m^)^sub app^ values for the α-helices are given in parentheses and the error on Φ was based on a uniform error in (τ^sup o^^sub m^) of 1
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22 µm Millipore (Billerica, MA) filter into a quartz cuvette and maintained at 25°C
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In general, we consider that DNA compaction is accompanied by dehydration of monocations and can proceed as through the decreasing of effective distance between DNA chains and through the changes in the parameters of DNA helix secondary structure
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It was shown in a seminal article by Semisotnov and co-workers that a blue shifted ANS fluorescence spectrum and a strongly increased quantum yield is diagnostic of binding to a molten-globule-like state with exposed hydrophobic patches (Semisotnov et al
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2) and transmission electron microscopy (Fig
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Our main goal with this study was to employ a variety of fluorescence probes (ANS, Bis-ANS, DCVJ, and ThT) and techniques (steady state and time resolved) to recognize and characterize different states of a protein in the process of misfolding and self-assembly
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Redetermination of the pressure dependence of the lipid bilayer phase transition
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The solutions appeared visually clear for temperatures at or above 130°C and a dissolving time of 2 hours, but small nuclei below the visible range may still have remained
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Specificity of the antibodies was examined by using the eggs shown in Table 2
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These distances are in agreement with the corresponding values obtained from the present spFRET experiments (see Results section above)
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Also weighing in was Rebecca Snead of the National Council of State Pharmacy Association Executives
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