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. The mutation will simply remain in the population
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. Moreover, resolution-enhancing techniques, such as second derivative and 2D IR, have been shown to be sensitive tools to monitor the sequential events in protein conformational changes by FTIR (27,28,30,38-42)
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. The amide I' hand of native ACYI in the original IR spectrum (Fig
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. The brief high temperature treatment of insulin results in a marked broadening of the height-distribution of the particles and its shift toward larger oligomers, which is shown in Fig
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. Regardless of the mechanism there should have been signs of greater atresia in the ovaries of young fish, which we did not observe in our histological preparations
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. Consistent with this hypothesis, previous trFRET studies have shown that the 4WJ stabilizes the tertiary structure of the hairpin ribozyme by reducing the entropie penalty for docking as compared with ribozyme constructs containing either a 2WJ or 3WJ (Klostermeier and Millar, 2000)
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. Journal of Raptor Research 25:140-141
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.9 ml/min^sup -1^, with intrinsic fluorescence detection at 315 nm (excitation at 280 nm)
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. It is worth noting that a multi-event equilibrium unfolding/ dissociation of human GR by GdnHCl has been previously reported (Nordhoff et al
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. For experiments involving PEP, control scans were acquired with PEP added to buffer versus buffer in the reference cell, and baselines were found to be the same as in buffer-buffer baselines, suggesting that under these conditions hydrolysis of PEP during heating was not significant
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. J Exp Med 2000; 191:515-528(18) Okazawa H, Motegi S, Ohyama N, et al
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. Octanol-Water Partition Coefficients: Fundamentals and Physical Chemistry
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.Model selection, order parameters, and timescales for internal motionBased on the qualitative analysis of the RT^sup n^^sub 1^ versus NOE plots, Fig
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. However, this same aspect leads to a lower affinity and specificity than for (cooperative) rigid binding (with all target sites simultaneously and rigidly interacting)
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. The increase in Na+ activity is rather steep compared to the moderate changes in activity between K+, Rb+, and Cs+
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. To better understand the basis for the observed broadening and tailing, we carried out numerical simulations using a simple model of the single-molecule FRET data
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