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. The enzymatic activity was determined by measuring the absorbance at 238 nm accompanied with hydrolysis of the substrate and using the molar absorption coefficient ε^sub 238^ = 185 M^sup -1^cm^sup -1^ as reported by Kordel and Schneider (5,6), except that chloroacetyl-L-leucine was used instead of pure-L-enantiomorph
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. Infrared spectroscopy The IR samples were prepared by dissolving ACYI in 100 mM deuterated phosphate saline buffer at a concentration of 50 mg/ml
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. Earlier studies have shown that this protein shows exceptionally high stability in terms of free energy of unfolding when compared to other proteins from the same family
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. 31nm and that of gSBA is ~4
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. The probability of linding a pair of residues in close spatial proximity decreases with increasing sequence separation
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. 6 K), all the measurements are within experimental error, and the differences are probably due to the broadness of the transition
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. The PTS system of Streptomyces coelicolor: identification and biochemical analysis of a histidine phosphocarrier protein HPr encoded by the gene ptsH
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. This is particularly intriguing, since its three-dimensional structure reveals that the active site of AChE, which, like other serine hydrolases, features a Ser-His-Glu catalytic triad, is buried at the bottom of a deep and narrow gorge lined by aromatic residues (26)
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. 2-4, the osmotic pressure determined using the osmotic stress technique is plotted as the dimensionless compressibility factor, Π/ρk^sub B^T, as a function of protein mass fraction for pH 8
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. Particle asymmetry also has an impact on the maximum packing fraction (64)
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. When comparing ThT and DCVJ fluorescence data from a protofibrillar sample of TTR (24 h incubation), it is evident that DCVJ shows pHindependent fluorescence (Fig
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. Deviations from the accurate field-theoretic exponent of v = 0
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