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Since gSBA and rSBA both exist as tetramers with identical ligand binding specificities and macromolecular properties, the change of the mode of unfolding from a two-state process to a three-state process and the decrease of T^sub m^ by 26°C for the thermal unfolding of the protein of the kilter implicate the covalenlly linked glycans for the stability of gSBA
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The structure of the modeled dimer nicely explained why the solvent accessibility of the Tip^sup 461^ was reduced as the concentration of EI^sup sc^ increased (see before)
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COPDApoptotic cells are increased in the lungs of COPD patients (3,8) and in animal models of COPD
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Resting and cytokine-stimulated human small airway epithelial cells recognize and engulf apoptotic eosinophils
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Biochemical characterization of Drosophila melanogaster acetylcholinesterase expressed by recombinant baculoviruses
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This indicates that the Bis-ANS molecule is too big to completely fit in the thyroxine binding cavity and likely there is only room for one-half of the symmetric molecule
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Abbreviations used: PLC, mammalian phosphoinositide-specific phospholipase C; PI(4,5)P^sub 2^, phosphatidylinositol 4,5- bisphosphate; POPC, 1-palmitoyl-2-oleoylphosphatidylcholine; POPS, 1-palmitoyl-2-oleoylphosphatidylserine; PE, 1-palmitoyl-2-oleoylphosphatidylethanolamine; PH, pleckstrin homology; DNS-PE, dansyl phosphadtiylethanolamine; FRET, fluorescence resonance energy transfer
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To determine whether this could be the case for PLCβ, we labeled the enzyme with CPM
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These mature HDL, which are further remodeled by plasma enzymes, form distinct subclasses differing in size, composition and metabolic properties (reviewed by Rye and Barter, 2004)
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Second, the heat-unfolding curve of the apoA-2:DMPC disks is sigmoidal and is shifted to higher temperatures as compared to that of free protein; thus the unfolding of free apoA-2 is complete at 80°C, well below the apparent melting temperature T^sub m^ = 84°C of the apoA-2:DMPC complexes observed at this scan rate (Fig
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, 2000; Rye and Barter, 2004; Jayaraman et al
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The thermodynamics of the self-association of the reduced and carboxymethylated form of apoA-II from the human high density lipoprotein complex
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A second feature is the potential for high cooperativity of structure in several simultaneous dimensions
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, 1985) and was towed vertically
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semifasciata has a quadrangulate anterior end and a margin almost straight, whereas the margin of the dentary in P
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Additional steps for removing solvent and dispersing agglomerated particles are required for producing separated polymer particles
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Postfertilization ovaries were very fragile and tended to rupture easily and release embryos under the slightest pressure
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In striking contrast, neither the 2WJ nor the 3WJ ribozymes can populate such a high-FRET state in the absence of loop A
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t] as described by (2) and (3) above
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. To better visualize the sequential events occurring during ACYI thermal unfolding, 2D IR synchronous and asynchronous plots were constructed
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. It was suggested, with the help of some computational studies, that this enhanced stability of the tetramer is mainly due to glycosylation and some stabilizing ionic interactions at the oligomeric interface (18)
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. Ice-induced ANS binding to other proteins To examine whether the ice-induced perturbation observed for azurin is common to proteins in general, ANS binding experiments were extended to a set of seven other proteins including monomeric BSA, α-amylase and β-gal, dimeric LADH and tetrameric ALD, YADH, and LDH
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.91 for aceturate, which agrees well with the respective values of 1
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.The R^sub 1^, R^sub 2^, and NOE data were analyzed using our new analysis method PINATA (22)
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. In proteins with more complex suctures, other candidates suggest themselves, such as the orientation of helices with respect to β-sheets with which they are in contact in α/β proteins, and the relative orientations of β-turns and partially-folded β-sheets in all-β elements
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