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Compared to native GR (circles), addition of 3 M GdnHCl (triangles) led to a significant increase in quenching efficiency, as indicated by an increase in K^sub SV^ from 12
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Protein was extensively dialyzed against 2 L of the working buffer (5 mM HEPES buffer containing 10 mM NaCl and 2 mM β-mercaptoethanol, pH 7) at 278 K
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The secondary structure of EI^sup sc^ upon binding was not largely affected (Table 4), but there were subtle changes in the stability of the protein (Table 3)
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Nuclear magnetic resonance imaging of a single cell
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0 M NaCl at pH 5
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