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The results in Fig
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Firstly, those residues making van der Waals clashes were selected and lilted with "Quick and Dirty" algorithms: and secondly, models were energy minimized (100 steps of steepest descent and 100 conjugate gradients, with a cut-off of 10 [Angstrom] for nonhonded interactions) with Insight II (Biosym/MSI)
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If a small substrate tilt angle remained, the height was measured twice, at both the cis- and transpositions
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The broad asymmetric peak shape observed for the SL 4WJ implies that extended undocked and quasi-docked states are in rapid conformational exchange, and our kinetic simulations indicate that the rate constant for formation of the quasi-docked state is in the range 10-41 × 10^sup 3^ s^sup -1^, whereas the corresponding reverse rate constant is in the range 3
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7 mg/mL^sup -1^ urea hydrogen peroxide and Tris buffer 0
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