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Taken together, these results suggest that at least two intermediate states can be identified in the equilibrium unfolding of GR, namely a partially unfolded predissociated state and a molten globule monomer
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Stock solutions of guanidinium hydrochloride were prepared in 5 mM HEPES buffer, pH 7
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For each of the sequences shown in Table 1
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For all cases, similar results were documented for experiments and modeling
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Hard spheres with surface adhesion: the Percus-Yevick approximation and the energy equation
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1 C'') A detailed description of all-or-none DNA compaction in a PEG-salt system as observed by FM is given in the literature (Minagawa et al
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The pleckstrin homology domain of phospholipase C-β^sub 2^ links the binding of Gβγ to the catalytic core
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Consequently, the high-temperature DSC transition involves apoA-2 unfolding
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It appears therefore that even for a homogenous insulin sample undergoing uniform temperature treatment, there is still more than one mode of superhelical assembly of protofilaments
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Although different distribution profiles of fibrillar features may still be explained in terms of kinetic effects (temperature may differently affect the kinetics at various stages of the assembly, effectively marginalizing certain sequential processes), the higher temperature may also have a more direct effect on amyloidogenesis, for instance by increasing the thermal energy of the interacting molecules and hence causing the alignment of protofilaments to become less accurate
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We need to articulate carefully what is meant in this context by "nonnative", for this term is sometimes used to refer to indiscriminate interactions
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Diffusional barrier crossing in a two-state protein folding reaction
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Length is a better predictor of fecundity than age as judged by the goodness-of-fit of the multiple linear regression model (Fig
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Sections were rehydrated and incubated in 200 µL hydrogen peroxide in 12 ml methanol to block the activity of endogenous peroxidase and then pre-treated in 0
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The OAO Severstal-owned Lucchini SpA steelworks of Italy also agreed to the same increase
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.1] = regression coefficients for the intercept and fork length, respectively
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